Identification of Mycobacterium kansasii by DNA hybridization
نویسندگان
چکیده
منابع مشابه
Detection of Mycobacterium kansasii Using DNA-DNA Hybridization with rpoB Probe
A microtiter well plate DNA hybridization method using Mycobacterium kansasii-specific rpoB DNA probe (kanp) were evaluated for the detection of M. kansasii from culture isolates. Among the 201 isolates tested by this method, 27 strains show positive results for M. kansasii, but the other 174 isolates were negative results for M. kansasii. This result was consistent with partial rpoB sequence a...
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Among biochemical tests useful in the study of mycobacteria, the hydroxylamine test is shown to be an effective tool in the differentiation of Mycobacterium kansasii from saprophytic strains of group III acid-fast bacilli when combined with the photochromogen test.
متن کاملIdentification of Mycobacterium kansasii by using a DNA probe (AccuProbe) and molecular techniques.
The newly formulated Mycobacterium kansasii AccuProbe was evaluated, and the results obtained with the new version were compared to the results obtained with the old version of this test by using 116 M. kansasii strains, 1 Mycobacterium gastri strain, and 19 strains of several mycobacterial species. The sensitivity of this new formulation was 97.4% and the specificity was 100%. Still, three M. ...
متن کاملIdentification of two groups of Mycobacterium paratuberculosis strains by restriction endonuclease analysis and DNA hybridization.
Genomic DNA was prepared from four reference strains of Mycobacterium paratuberculosis and 46 isolates of this organism from New Zealand, Australia, Canada, and Norway and also from two mycobactin-dependent "wood pigeon" strains. The DNA was characterized by restriction endonuclease analysis, both with and without DNA hybridization, with a probe specific to a repetitive DNA sequence in M. parat...
متن کاملAssessment of morphology for rapid presumptive identification of Mycobacterium tuberculosis and Mycobacterium kansasii.
Mycobacterium tuberculosis often exhibits serpentine cording when grown in liquid medium, whereas Mycobacterium kansasii can be larger and cross-barred. We assessed the use of these morphologic characteristics as a cost-effective method for rapid presumptive identification of isolates from BACTEC bottles. Without specific training, using the Kinyoun acid-fast stain, definitive cording was found...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1991
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.29.10.2125-2129.1991